토마토황화잎말림바이러스 저항성 검정용 멀티플렉스 PCR 프라이머 세트

Fecha de publicación: 13/04/2018
Fuente: Wipo tomato
The present invention relates to a multiplex PCR primer set for detecting resistance against tomato yellow leaf curl virus. More specifically, the present invention relates to a primer set comprising seven kinds of primers for detecting resistance against tomato yellow leaf curl virus by detecting, through multiplex PCR, a specific molecular marker related to Ty2 and Ty3 genes in the tomato genome; a composition comprising the primer set; a kit; and a method for detecting resistance against tomato yellow leaf curl virus by using the primer set. According to the present invention, through a single multiplex PCR reaction, molecular markers related to resistance against tomato yellow leaf curl virus can be accurately detected in various tomato varieties and strains. In particular, by resolving the problem of an existing method which generates an unexpected PCR amplification product, depending on tomato varieties and strains, that causes errors in the detection of resistance, the present invention enables an efficient and reliable detection of resistance in various tomato varieties and strains. Accordingly, the present invention is expected to greatly contribute to the development of novel superior tomato varieties. COPYRIGHT KIPO 2018