Fuente: PubMed "Tobacco production" Pestic Biochem Physiol. 2026 Mar;218:106967. doi: 10.1016/j.pestbp.2026.106967. Epub 2026 Jan 18.ABSTRACTTobacco black shank (TBS) is a soil-borne disease caused by Phytophthora nicotianae (P. nicotianae), which poses a significant threat to both the yield and quality of tobacco crops. Biocontrol bacteria play a vital role in sustainable agriculture, including the management of TBS. Consequently, finding biocontrol bacteria that effectively inhibit P. nicotianae is important for tobacco production. In this study, a biocontrol bacterium with a promising control effect on TBS was isolated from the rhizosphere soil of tobacco plants, and named BY-S1. BY-S1, identified as Bacillus subtilis, exhibited an antagonistic effect of 70.60% against P. nicotianae, along with the capabilities for phosphate solubilization and siderophore production. Furthermore, an indoor potted plant experiment was conducted to explore the effect of BY-S1 on tobacco growth and resistance. The results showed that BY-S1 not only promoted tobacco growth, but also significantly reduced the TBS incidence, reaching a control efficacy of 53.45%. BY-S1 application enhanced photosynthesis, increased the activities of antioxidant enzymes (SOD, POD and CAT) and defense enzymes (PPO and PAL), and regulated the contents of plant hormones (IAA, ABA, JA and SA) to improve tobacco resistance against TBS. In addition, BY-S1 also protected tobacco from P. nicotianae infections by modulating metabolisms such as ascorbate and aldarate metabolism and tryptophan metabolism. These findings highlight the potential of BY-S1 in controlling P. nicotianae infection in tobacco.PMID:41629035 | DOI:10.1016/j.pestbp.2026.106967