Fecha de publicación: 08/07/2022 Fuente: Wipo Tobacco Agriculture The invention discloses a tobacco seedling stage bacterial wilt resistance identification method, which belongs to the technical field of plant disease resistance identification, and comprises the following four steps: transplanting floating seedlings in a small cross period, inoculating ralstonia solanacearum in a high-concentration fixed plant to construct a micro disease nursery after standardized root system minimally invasive in a large cross period, inducing diseases at high temperature and high humidity, and investigating and evaluating the resistance of systematic diseases. According to the method, bacterial wilt resistance identification and floating seedling raising are fused together, the method has the advantages of being programmed in operation, capable of reducing cost, saving space and labor, high in selection efficiency, accurate, stable and high in comparability in tobacco bacterial wilt resistance phenotype identification and the like, the process from sowing to identification completion can be completed generally in two months, the timeliness and scale of tobacco bacterial wilt resistance identification are improved, and the method is suitable for large-scale popularization and application. The method is worthy of popularization.