Codominant SSR molecular marker closely linked with type III tobacco sucrose ester gene qBMVSE471 and application of codominant SSR molecular marker

Fecha de publicación: 04/01/2022
Fuente: Wipo Tobacco Agriculture
The invention discloses a codominant simple sequence repeat(SSR) molecular marker closely linked with a type III tobacco sucrose ester gene qBMVSE471 and application of the codominant SSR molecular marker. The serial numbers of the codominant SSR molecular marker closely linked with the type III tobacco sucrose ester gene qBMVSE471 are TMc42726 and TMt09661, and nucleotide sequences of polymerase chain reaction (PCR) amplification products of the codominant SSR molecular marker closely linked with the type III tobacco sucrose ester gene qBMVSE471 are respectively as shown by SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The invention further discloses application of the codominant SSR molecular marker closely linked with the type III tobacco sucrose ester gene qBMVSE471 to detecting whether the type III tobacco sucrose ester gene qBMVSE471 is present in tobacco genome deoxyribose nucleic acid (DNA) or not. The codominant SSR molecular marker closely linked with the type III tobacco sucrose ester gene qBMVSE471 provided by the invention has the characteristics of accuracy, high efficiency, stability, convenience and low cost, and therefore the molecular marker can be applied to III type sucrose ester gene qBMVSE471 molecular marker-aided selection in tobacco high-aroma quality breeding.