Fecha de publicación: 28/01/2022 Fuente: Wipo Tobacco Agriculture The invention relates to a codominant SSR marker closely linked with an IV-type tobacco sucrose ester gene qBMVSE485 and application of the codominant SSR marker. The serial numbers of the codominant SSR marker closely linked with the IV-type tobacco sucrose ester gene qBMVSE485 are TM50931 and TM56908, TMc44058 and TMc44049, and the nucleotide sequences of PCR amplification products of the codominant SSR marker closely linked with the IV-type tobacco sucrose ester gene qBMVSE485 are respectively shown as SEQ ID No.1 and SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6, SEQ ID No.7 and SEQ ID No.8. the application is the application of the codominant SSR marker closely linked with the IV type tobacco sucrose ester gene qBMVSE485 in detecting whether the IV type tobacco sucrose ester gene qBMVSE485 exists in a tobacco genome DNA or not. The codominant SSR marker disclosed by the invention has the characteristics of accuracy, high efficiency, stability, convenience and low cost, so that the molecular marker can be applied to molecular marker-assisted selection of the IV type sucrose ester gene qBMVSE485 in tobacco high-aroma-quality breeding.