Fuente: WIPO "tobacco agriculture" The invention discloses a biocontrol strain NYK122 for preventing and treating aromatic tobacco sickle type fusarium wilt and anti-biological inoculant of the biocontrol strain NKY122, belonging to the technical field of bio-pesticides. The preservation number of the strain NKY122 is CGMCC (China general Microbiological Culture Collection) NO.7722. The anti-biological inoculant is obtained by the following steps of transplanting the strain NYK122 to a test tube for activating, culturing for 48 hours at 35 DEG C, transferring the activated lawn into 800ml of NA culture solution which is contained in a 1000ml triangular flask, culturing for 48 hours at the rotation speed of 150 revolutions/minute under the temperature of 35 DEG C to obtain primary fermentation liquor; inoculating the primary fermentation liquor into the NA culture solution in a seed tank according to an inoculation amount proportion of 1:200, thereby obtaining strain liquor for secondary fermentation after fermenting for 48 hours with the temperature of the seed tank of 35 DEG C-37 DEG C; and inoculating the strain liquor for the secondary fermentation to a fermentation tank according to the inoculation amount proportion of 1:20, fermenting for 96 hours at the temperature of 35 DEG C-37 DEG C, and storing the strain liquor as biocontrol liquor, centrifuging for 5 minutes at 2000 rpm, and preparing with special-purpose preservative fluid to form fungicide, so that the total concentration of viable bacteria in the fungicide is 1*1011 CFU/ml to 1*1012 CFU/ml. According to the biocontrol strain NYK122 for preventing and treating aromatic tobacco sickle type fusarium wilt and the anti-biological inoculant of the biocontrol strain NKY1, the cost is low, the aromatic tobacco sickle type fusarium wilt can be effectively prevented, and the yield and the quality of the aromatic tobacco are improved.