ISHS (International Society for Horticultural Science) Post date: Friday 10 June 2022Fecha de publicación: 10/06/2022 Fuente:
Chian-Chi Lin is an undergraduate student of the Department of Medical Laboratory Science and Biotechnology, Asia University, Wufeng, Taichung, Taiwan. Her research is addressed to identify a new virus affecting amaryllis (Hippeastrum hybridum Hort.), which is a perennial bulbous plant belonging to the Amaryllidaceae family very popular as garden flower in Taiwan. The ornamental value of amaryllis plants can be greatly affected by several viruses. In Taiwan, Capsicum chlorosis virus (CaCV), Cucumber mosaic virus (CMV), Hippeastrum mosaic virus (HiMV) and Nerine latent virus (NeLV) have been already shown to infect amaryllis. In April 2011, we observed amaryllis showing chlorotic ringspots at a private garden in Changhua County, central Taiwan. The virus was obtained by single lesion isolation on Chenopodium quinoa leaves. Filamentous virus particles of about 1000 nm long were examined in the crude sap of symptomatic plant tissue by transmission electron microscopy. Total RNA was extracted from the virus-infected C. quinoa leaves as a template to build a cDNA library using random primers. The Illumina MiSeq sequencing system was used to obtain the genomic sequences of the virus. Sequence reads were de novo assembled to generate contigs and then were subjected to BLAST searches against the non-redundant protein database of NCBI for annotation. A 9377 nt contig consisting of a putative open reading frame of a potyviral polyprotein was obtained. It shares 95.5% nt identity and 96.5% aa identity with the partial genome sequence of Amazon lily mosaic virus (ALiMV) (NC_043137.1). The complete genome sequence of the virus was clarified by rapid amplification of cDNA ends. Genome sequence analysis indicated that the amaryllis virus is a distinct potyvirus ALiMV. To our knowledge, this is the first report on ALiMV infecting amaryllis in Taiwan.
Chian-Chi Lin won the ISHS Young Minds Award for the best poster presentation at the XV International Symposium on Virus Diseases of Ornamental Plants, which was held virtually in USA and Singapore in December 2021.
Chian-Chi Lin, Department of Medical Laboratory Science and Biotechnology, Asia University, Wufeng, Taichung, Taiwan, No. 500, Liufeng Rd., Wufeng Dist., Taichung City 413305, Taiwan (R.O.C.), e-mail: email@example.com
The article is available in Chronica Horticulturae
Tags: virus diseases of ornamental plantsCategories: Young Minds Award Winners