PROCESS FOR PRODUCING A TAN IIA NANOLIPOSOME SYSTEM FOR FOODS AND MEDICAL PRODUCTS

Fecha de publicación: 08/07/2021
Fuente: Wipo "olive oil"
The invention relates to a process for producing a Tan IIA nanoliposome system for foods and medical products, comprises: (i) preparing a dispersed phase by dissolving Tan IIA in ethanol in a Tan IIA weight:ethanol volume ratio of 8:10 by a stirrer at 300-500 rpm with heating to 40-60° C. within 4-8 hours; (ii) preparing a liposome carrier consisting of lecithin and olive oil in a ratio of 1:3 by weight by mixing in a constant temperature bath at 40-60° C. to ensure that lecithin is completely dissolved in the oil while stirring; (iii) adding the carrier to the dispersed phase in a ratio of 40:60 by weight, further heating the carrier and dispersed phase mixture to 40-60° C. and stirring at 800-1000 rpm within 1 to 2 hours; (iv) cooling the resulting mixture to 25° C. and pumping the cooled mixture, using an ultrasonic atomizer nozzle at 60 Hz (10-20 μm droplet size, 10 mL/min), into 1-1.5 L of distilled water (with the temperature of the distilled water at 25° C.) to obtain a liposome suspension-water mixture; (v) homogenizing the liposome suspension-water mixture by pumping through a high pressure homogenizer at 30 Mpa to obtain a Tan IIA nanoliposome system as a homogeneous and stable mixture with a confirmed particle size smaller than 200 nm.