Evaluation of Cardioprotective Activity of flowers of Butea monosperma against Isoproterenol induced Cardiac Hypertrophy in Wistar Albino Rats

Fecha de publicación: 08/01/2021
Fuente: Wipo "olive oil"
AbstractThe objective of the investigate the effect of pretreatment with Butea monosperma on isoproterenol (ISO)-induced cardiotoxicity and cardiac hypertrophy in rats. Methods: Albino male Wistar rats (250–300 g) were evenly divided into following groups: Control group (2% acacia solution (2ml/kg p.o.) orally for 18 days and water IP from days 9–18). ISO group (olive oil 2 mL/kg orally for 18 days and ISO 1 mg/kg IP from days 9–18); BM (300mg/kg)+ ISO: Animals were administered with BM (300mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). BM (600mg/kg) + ISO: Animals were administered with BM (600mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). Co Q10 (100mg/kg) + ISO: Animals were administered with Co Q10 (100mg/kg p.o.) for 18 days and injected with Isoproterenol from 9th day (1ml/kg IP). And the twenty-four hours after the last dose of water or ISO, the rats were anesthetized, and an ECG was recorded. Blood was withdrawn by retro-orbital puncture for estimation of serum creatine kinase-MB (CK-MB) isoenzyme levels, lactate dehydrogenase (LDH) levels, and aspartate aminotransferase activities. The animals were euthanized using an overdose of ether. The hearts of 6 animals from each group were used for estimation of superoxide dismutase (SOD) activity, reduced glutathione (GSH) concentration, lipid peroxidation (LPO), malondialdehyde (MDA), and total protein concentration.