Fecha de publicación: -- Fuente: PubMed "plant biotechnology" Nanomaterials (Basel). 2026 Sep 20;16(18):1185. doi: 10.3390/nano16181185.ABSTRACTIn order to investigate the effects of carbon dots on Siraitia grosvenorii tissue culture, fluorescent carbon dots (L-CDs) were prepared via a one-step hydrothermal method using S. grosvenorii leaves as a novel carbon source. The synthesized L-CDs exhibited blue fluorescence under UV irradiation, with an optimal excitation wavelength of 379 nm and an optimal emission wavelength of 451 nm. The L-CDs demonstrated excellent biocompatibility, superior anti-photobleaching and salt-tolerance properties, and high stability across a wide pH range of 1-12, making them highly suitable for plant tissue culture. Notably, 0.6 mg·L-1 L-CDs significantly promoted the growth of S. grosvenorii tissue-cultured seedlings and increased their propagation coefficient. Further studies revealed that 0.6 mg·L-1 L-CDs significantly enhanced antioxidant enzyme activities, with SOD, POD, and CAT activities increasing by 82.61%, 33.33%, and 58.82%, respectively, compared to the control group. This study highlights the potential of L-CDs as highly efficient and non-toxic growth promoters in plant tissue culture, providing a sustainable and effective strategy for their application in plant biotechnology.PMID:42784193 | DOI:10.3390/nano16181185