Fecha de publicación: -- Fuente: PubMed "essential OR oil extract" J Neurosci. 2026 Sep 24:e0240262026. doi: 10.1523/JNEUROSCI.0240-26.2026. Online ahead of print.ABSTRACTGliogenesis is a multistep process in which glial progenitors are specified into migrating and proliferating precursor cells that subsequently differentiate into mature astrocytes and oligodendrocytes. How these developmental processes are coordinated to generate the diverse glial lineages across gray matter (GM) and white matter (WM) remains poorly understood. Here, we show that the basic-helix-loop-helix (bHLH) transcription factor ASCL1 serves as a direct mechanistic link between these processes in the dorsal forebrain. Notably, ASCL1 is dynamically expressed in glial progenitor cells, initiating in the ventricular zone (VZ), peaking in the intermediate zone (IZ), but is downregulated once glial cells enter the cortical plate. Lineage tracing of ASCL1+ progenitors demonstrates that they subsequently co-express OLIG2 to generate both astrocytes and oligodendrocytes in an "outside-in" pattern starting from the upper cortex inward to the corpus callosum, the opposite pattern of neurogenesis. ASCL1 gain-of-function combined with OLIG2 loss-of-function reveals that sustained levels of ASCL1 are essential to induce sufficient levels of OLIG2 required to specify oligodendrocyte precursor cell (OPC) fate. Interestingly, a persistent ASCL1 expression also maintains OPCs in an undifferentiated, pre-neoplastic state into postnatal stages, in part by suppressing their differentiation into postmitotic oligodendrocytes. Together, these findings establish ASCL1 as a key regulator of the spatiotemporal order of glial lineage diversity in cortical GM and callosal WM, and highlight ASCL1 dysregulation as an underlying developmental mechanism in the pathogenesis of gliomas.Significance statement The brain relies on macroglia to form the blood-brain-barrier, insulate nerve fibers, and regulate neuronal signaling; yet how these cell types are generated remains poorly understood. This study shows that the timing and levels of two key regulatory proteins, ASCL1 and OLIG2, function together to guide immature glial cells through distinct stages of development. Notably, we show that brief ASCL1 activity followed by sustained ASCL1 and OLIG2 levels coordinate when glial cells form, the cell type they become, and where they migrate to reside in the cerebral cortex. By revealing the developmental program underlying the generation of glial cell diversity, this study provides a framework for understanding how glial-related neurological diseases and cancer arise in the brain.PMID:42785991 | DOI:10.1523/JNEUROSCI.0240-26.2026