Fecha de publicación: 10/05/2022 Fuente: WIPO "royal jelly" The invention provides royal jelly zymolyte with a liver protection effect and a preparation method of the royal jelly zymolyte, and belongs to the field of biological medicines. The method comprises the following steps: step 1, royal jelly is subjected to self-enzymolysis, and the self-enzymolysis conditions are as follows: royal jelly and water are mixed and then react for 1-8 hours under the conditions that the temperature is 25-45 DEG C and the pH value is 4-8, and self-enzymolysis liquid is obtained; and 2, adding an exogenous enzyme into the self-enzymatic hydrolysate, and carrying out enzymolysis to obtain the royal jelly zymolyte. The royal jelly zymolyte can effectively help liver cells to resist oxidative stress injury caused by hydrogen peroxide, protect the liver cells and treat and prevent liver diseases, and provides a novel pharmaceutical ingredient; the royal jelly endogenous enzyme is used for enzymolysis, the royal jelly activity is improved, and a new direction is provided for full development and utilization of royal jelly resources; the special enzymolysis method and the special restriction enzyme cutting site selection endow the royal jelly zymolyte with new functional activity, significantly improve the product value, and provide a new way for the production and transformation of the royal jelly zymolyte.