Fecha de publicación: 04/09/2020 Fuente: WIPO "propolis" ABSTRACTA METHOD FOR COMPOSITION OF AN INDUCER USING INDIAN PROPOLIS AND IT’S USE FOR DIFFERENTIATION OF HUMAN DENTAL PULP STEM CELLS INTO EPITHELIAL AND NEURAL-LINEAGESA method for composition of an inducer using Indian propolis and differentiation of dental pulp stem cells into epithelial and neural cells using inducer is provided. method comprises steps of procuring Indian propolis and air drying Indian propolis, crushing dried Indian propolis thereby forming power of Indian propolis, macerating powder in of solution of ethanol , filtering macerated power and forming ethanolic extract of Indian propolis thereby obtaining inducer, extracting teeth and placing teeth onto hank’s solution, sectioning teeth to retrieve pulp inside teeth, mixing pulp in predetermined quantity of FBS thereby obtaining pulpal tissue, mincing and treating pulpal tissue with of collagenase and of Dispase , shaking and centrifuging mixture and separating supernatant from mixture thereby forming pellet from mixture, suspending pellet in an aMEM and forming mixture, centrifuging mixture and removing supernatant formed from mixture, seeding pellet with aMEM, Glutamine, antibiotics penicillin, streptomycin and 1 to 20% FBS solution, rinsing confluent cells in phosphate buffered saline (PBS), mixing with of dispase and of enzyme solution thereby forming mixture and shaking mixture to form supernatant, propagating supernatant in a-MEM containing of FBS and incubated at predetermined temperature with of CO2 Incubator thereby obtaining hDPSCs, incubating hDPSCs with of inducer diluted with a-MEM containing of FBS thereby obtaining epithelial cells and neural cells.[FIGURE 1]