Method for identifying cysticercosis resistance character of bee colony by utilizing SNP marker KZ288474.1_322717

Fecha de publicación: 02/03/2021
Fuente: WIPO "apis cerana"
The invention relates to a method for identifying swarms cysticercosis resistance traits by using SNP (single nucleotide polymorphism) markers KZ288474.1_322717. The method comprises the following steps of bee larva individual sampling, bee larva sample DNA (deoxyribonucleic acid) extraction, primer synthesis, PCR (polymerase chain reaction) detection and identification of Chinese bee cysticercosis resistance traits; And identifying the cysticercosis resistance traits of the apis cerana according to the fact that whether obvious difference exists between the frequency PG of the G gene appearing in the SNP marker KZ288474.1_322717 and the frequency PC of the C gene appearing in the apis cerana larva individuals randomly collected from the swarms. From the molecular level, the invention provides the SNP (KZ288474.13_22717) marker related to the cysticercosis resistance of the worker bee larvae of the apis cerana, the cysticercosis resistance of the apis cerana is scientifically, accurately and quickly identified, the breeding period of the apis cerana resisting the cysticercosis can be greatly shortened, and the breeding speed of the apis cerana is increased.