Fecha de publicación: 10/07/2020 Fuente: WIPO "apis cerana" The invention relates to a method for detecting apis mellifera and apis cerana honey glucose dehydrogenase through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: selecting characteristic peptide fragments respectively belonging to glucose dehydrogenase of apis mellifera honey and apis cerana honey, establishing a liquid chromatography-tandem mass spectrometry detection method, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection and performing data analysis, wherein the characteristic peptidefragments comprise an apis mellifera glucose dehydrogenase characteristic peptide fragment, namely ITLDNQSVQVR, and an apis cerana glucose dehydrogenase characteristic peptide fragment, namely FPYQPPFAWEILK. The method for detecting glucose dehydrogenase in honey has high accuracy, precision and sensitivity and strong stability and is suitable for distinguishing apis mellifera honey from apis cerana honey and is especially suitable for identifying apis mellifera honey doped in apis cerana honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.