Method for constructing fingerprint spectrums of natural Apis cerana longan honey and natural A.mellifera ligustica longan honey and identification and application of fingerprint spectrums ofnatural Apis cerana longan honey and natural A.mellifera ligustica longan honey

Fecha de publicación: 15/03/2019
Fuente: WIPO "apis cerana"
The invention discloses a method for constructingfingerprint spectrums of natural Apis cerana longan honey and natural A.mellifera ligustica longan honey and identification and application of the fingerprint spectrums of the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey. A headspace solid-phase microextraction-gas chromatography-mass spectrometry method and aChinese herbal fingerprint spectrum similarity evaluation system are used to respectively construct GC/MS fingerprint spectrums of the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey; the GC/MS fingerprint spectrum of the natural Apis cerana longan honey is determined to contain 16 common feature volatile ingredients, and the GC/MS fingerprint spectrum of thenatural A.mellifera ligustica longan honey contains 15 common feature volatile ingredients. By comparing the similarity and the difference of the chemical composition of a total ion chromatogram of a to-be-tested natural longan honey sample and the constructed GC/MS fingerprint spectrum, the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey are accurately distinguished and identified. The method has the characteristics of small use level of samples, strong characteristics and reliable result, is quite beneficial for solving the problem that the Apis cerana longanhoney and the A.mellifera ligustica longan honey are difficult to distinguish on the market, and has a quite remarkable social benefit.