Fecha de publicación: 25/10/2019 Fuente: WIPO "apis cerana" The invention provides an ELISA detection kit for detecting Apis cerana Smith honey and Apis mellifera honey. The MRJP2(major royal jelly protein 2) is selected as the target protein for Apis cerana Smith honey and Apis mellifera honey detection, the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2 are obtained through a Prokaryotic expression purification, and preparing and purifying to obtain the specificity and identity antibody of the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2, and the Apis cerana Smith honey and the Apis mellifera detection kit is developed by using a doubleantibody sandwich method. The ELISA detection kit disclosed by the invention has high sensitivity, the developing condition can be observed through the naked eyes, 1% of Apis mellifera honey doping in the Apis cerana Smith can be identified within 45min, the convenient and fast detection requirement is satisfied, and the detection kit is a reliable tool for Apis cerana Smith honey and Apis mellifera honey detection.