Changbai mountain apis cerana SSR marker primer group, PCR identification method and application

Fecha de publicación: 23/07/2021
Fuente: WIPO "apis cerana"
The invention discloses a Changbai mountain apis cerana SSR marker primer group, a PCR identification method and application, and relates to the technical field of molecular biology and bioinformatics. Aiming at the apis cerana population, an apis cerana SSR marker primer is developed based on three-generation full-length transcriptome sequencing, and the marker primer has the advantages of rapidness, high efficiency, large number of developed markers, high availability and the like, and can be directly applied to analysis of genetic diversity of the Changbai mountain apis cerana population and conventional apis cerana population; and technical support is provided for follow-up cold resistance evaluation and identification of apis cerana germplasm resources, molecular-assisted breeding of low-temperature-resistant bee varieties, construction of genetic maps, correlation analysis of cold resistance characters, mining of functional genes, genetic evolution and the like, and the maker primer group has good application prospects and scientific research value.