Fuente: WIPO "essential oil or extracts" Present subject matter relates to a method (600) for analysis of synergistic 5 anti-fungal composition of essential oil extracted from leaves of Callistemon viminalis with antibiotics comprises performing surface sterilization of the leaves of Callistemon viminalis. Further the method comprises extracting essential oil from the leaves of Callistemon viminalis. Further, the method comprises preparing synergistic anti-fungal composition from the extracted essential oil with antibiotics. 10 Further, the method comprises performing one or more analysis of the synergistic anti-fungal composition by broth dilution method. Thereafter, the method discloses an output indicating indicating the extracted essential oil from the leaves of Callistemon viminalis which is a potent formulation in the synergistic anti-fungal composition for treating drug resistant Candida based fungal infections, and thereby 15 reduces dosage of the antibiotics by 3 to 34 folds.